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1. ÇöÁ¸ÇÏ´Â Ç×ü°íÁ¤ ¹®Á¦Á¡ ¹× ¿ä±¸±â¼ú
Strategies of Antibody Immobilization
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2. Ç×ü°íÁ¤ Á¦¾î ½Å±â¼ú
A) With biotin-BMCC only, reaction occurrence in prior
at more accessible sites (hinge < inter-H&L chains)
B) In the presence of free maleimide in addition to biotin-BMCC,
¡æ Competitive reaction of these two species for the sulfhydryl moieties at
the inter-H&L chains (site 1). Coupling of biotin BMCC to the sulfhydryl groups at the hinge region (site 2).
Ç×ü°íÁ¤ Á¦¾î±â¼ú °³¹ß
Âü°í¹®Çå I. H. Cho, E. H. Paek, H. Lee, J. W. Choi, and S. H. Paek.
(2004) Site-directed immobilization of antibody onto solid surfaces for
the construction of immunochip. Biotech. Biopr. Eng. 9, 112-117. |
3. Ç×ü°íÁ¤ ±â¼úÀÀ¿ë
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